《植物生理学报》 2010, 46(12): 1237-1242

柽柳bZIP 基因调控耐盐相关基因的表达

吴英杰, 王超, 及晓宇, 王留强, 王玉成＊

东北林业大学林木遗传育种与生物技术教育部重点实验室, 哈尔滨150040

通信作者：王玉成；E-mail: wangyucheng1029@126.com；Tel: 0451-82191627

摘　要：

本研究探讨了柽柳(Tamarix hispida) bZIP (basic leucine zipper)基因对抗逆基因表达的调控。我们比较了非盐胁迫和 盐胁迫条件下非转基因和转 bZIP 基因植株的 SOD、POD、ATPase、GST、LTP 和 LEA 等基因表达量的变化。结果表明, 在非胁迫条件下, bZIP 转录因子可能直接调控了 poxN1、TOBPXD、TOBLTP 和 ltp1 基因的表达, 而其他基因的表达可能 不受 bZIP 转录因子的直接调控。在盐胁迫下, bZIP 转录因子能够直接或间接地调控部分抗逆基因的表达, 使它们的表达 量显著增强。

关键词：bZIP 基因; 烟草; 盐胁迫; 实时定量 RT-PCR; 基因表达

收稿：2010-09-17   修定：2010-11-03

资助：国家自然科学基金(30972387)

Expression of Salt-Tolerant Genes Regulated by a bZIP Gene in Tamarix hispida Willd

WU Ying-Jie, WANG Chao, JI Xiao-Yu, WANG Liu-Qiang, WANG Yu-Cheng*

Key laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University, Ministry of Education, Harbin 150040, China

Corresponding author: WANG Yu-Cheng; E-mail: wangyucheng1029@126.com; Tel: 0451-82191627

Abstract:

The genes whose expression regulated by a basic leucine zipper (bZIP) gene from Tamarix hispida were investigated in the present study. Expression of stress-tolerant genes including SOD, POD, ATPase, GST, LTP and LEA were compared between bZIP transformed and non-transgenic plants under normal growth and salt stress condition. The results showed that under normal condition, poxN1, TOBPXD, TOBLTP and ltp1 genes may be directly regulated by bZIP, while the others may be not regulated by bZIP directly. Under salt stress bZIP regulated the expression of some stress resistance genes and enhance their expression level.

Key words: bZIP; tobacco; salt stress; real-time PCR; gene expression